HSP27 Antibody

• Catalog Number: orb323172
• Category: Antibodies
• Description: The HSP27 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human HSP27. Purification: Affinity chromatography
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: Recombinant protein corresponding to human HSP27.
• UniProt ID: P04792
• Tested applications: IHC, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:100-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSP27; HSP28; Heat shock protein beta-1; HspB1; 28
• Research Area: Epigenetics, Infectious Diseases, Neuroscience
• Note: For research use only
• Entrez: 3315
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HSP27 expression in Hela (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.