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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, ICC, IHC-Fr, IP |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 01G |
| Immunogen | HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| Target | HLA-G |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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HLA-G Antibody [orb2309990]
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Mouse
Monoclonal
Unconjugated
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Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified antibody (concentration in sample 16 µg/ml) GAM APC.
Flow cytometry surface staining pattern of HLA-G transfected LCL cells using anti-human HLA-G (01G) purified antibody (concentration in sample 16 µg/ml) GAM APC.
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Protocol Information
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
HLA-G Antibody (orb43572)
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