Anti-HLA Class I PE

• Catalog Number: orb44057
• Category: Antibodies
• Description: Mouse monoclonal antibody against HLA-A conjugated to PE
• Target: HLA Class I
• Clonality: Monoclonal
• Isotype: Mouse IgG3
• Conjugation: PE
• Reactivity: Bovine, Human, Primate
• Concentration: 0.1 mg/ml
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: COS-7 African green monkey kidney cells
• RRID: AB_10999453
• Tested applications: FC
• Application notes: Flow cytometry: Recommended dilution: 2-4 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: MEM-123
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: Anti-HLA A Antibody, anti-HLA B Antibody, anti-HLA
• Note: For research use only

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 µg/ml).

Separation of human lymphocytes (red-filled) from HLA Class I negative CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 µg/ml).

Separation of human lymphocytes (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood spiked with K562 cells stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 µg/ml).

Flow cytometry surface staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 µg/ml).