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Featured
Description
Research Area
Protein Biochemistry
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:3000, IF/ICC: 1:100-1:200, IP: 1:50-1:200 |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of His-tag. The exact sequence is proprietary. |
| Purification | Affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation







![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256371_1.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256371_2.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256371_3.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256374_1.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256374_2.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256374_3.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256375_1.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256375_2.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256375_3.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256376_1.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256376_2.png)
![Anti-His tag (C-term) [3D5]](/images/pub/media/catalog/product/NewWebsite/35/orb256376_3.png)