Anti-His-tag Antibody

• Catalog Number: orb323049
• Category: Antibodies
• Description: Mouse monoclonal antibody to His-tag
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of His-tag. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000-1:3000, IF/ICC: 1:100-1:200, IP: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of over-expressed His-tagged protein in 293T cell lysate. (Predicted band size: \; Observed band size: \)

Immunofluorescent analysis of His-tag staining in 293T cells transfected with a His-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of His-tagged protein from HEK293T cells transfected with vector overexpressing His tag, using Anti-His-tag Antibody.