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His Tag Antibody (monoclonal, 19b10)
Catalog Number: orb548525
| Catalog Number | orb548525 |
|---|---|
| Category | Antibodies |
| Description | Anti-His Tag Antibody (monoclonal, 19b10). Tested in WB application. This antibody reacts with all species. |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG2b |
| Conjugation | Unconjugated |
| Reactivity | All |
| Form/Appearance | Lyophilized |
| Immunogen | A synthetic peptide corresponding to a sequence of His Tag. |
| UniProt ID | P50150 |
| MW | 12 kDa |
| Tested applications | WB |
| Application notes | Western blot, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500μg/ml |
| Antibody Type | Primary Antibody |
| Clone Number | 19b10 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ATP synthase D chain mitochondrial antibody; ATP s Read more... |
| Note | For research use only |
Western blot analysis of His Tag using anti-His Tag antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse His Tag protein 10 ng, Lane 1: recombinant mouse His Tag protein 5 ng, Lane 1: recombinant mouse His Tag protein 2.5 ng, Lane 1: recombinant mouse His Tag protein 1.25 ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
