Anti-His Tag Antibody (monoclonal, 19b10)

• Catalog Number: orb548525
• Category: Antibodies
• Description: Anti-His Tag Antibody (monoclonal, 19b10). Tested in WB application. This antibody reacts with all species.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 19b10
• Tested applications: WB
• Reactivity: All
• Isotype: Mouse IgG2b
• Immunogen: A synthetic peptide corresponding to a sequence of His Tag.
• Antibody Type: Primary Antibody
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 12 kDa
• UniProt ID: P50150
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ATP synthase D chain mitochondrial antibody; ATP s
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500μg/ml
• Expiration Date: 12 months from date of receipt.

Western blot analysis of His Tag using anti-His Tag antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse His Tag protein 10 ng, Lane 1: recombinant mouse His Tag protein 5 ng, Lane 1: recombinant mouse His Tag protein 2.5 ng, Lane 1: recombinant mouse His Tag protein 1.25 ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.