GNG4 Mouse Monoclonal Antibody

SKU: orb1145789

Description

Anti-GNG4 Antibody (monoclonal, 7B6). Tested in WB applications. This antibody reacts with Mouse, Rat.

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 7B6
• Immunogen: E.coli-derived human GNG4 recombinant protein (Position: M1-D52).
• Target: Guanine nucleotide-binding protein G
• Molecular Weight: 12 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP synthase D chain mitochondrial antibody; ATP synthase H+ transporting mitochondrial F1F0 subunit antibody; ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody; ATP synthase subunit d antibody; ATP synthase subunit d, mitochondrial antibody; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody; ATP5H antibody; ATP5H_HUMAN antibody; ATP5JD antibody; ATPase subunit d antibody; ATPQ antibody; mitochondrial antibody; My032 protein antibody

Western blot analysis of GNG4 using anti-GNG4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNG4 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12 kDa. The expected band size for GNG4 is at 12 kDa.

Quick Database Links

Gene Symbol

Guanine nucleotide-binding protein G

UniProt

P50150