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Anti-GNG4 Antibody (monoclonal, 7B6)
Catalog Number: orb1145789
| Catalog Number | orb1145789 |
|---|---|
| Category | Antibodies |
| Description | Anti-GNG4 Antibody (monoclonal, 7B6). Tested in WB applications. This antibody reacts with Mouse, Rat. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 7B6 |
| Tested applications | WB |
| Reactivity | Mouse, Rat |
| Isotype | Mouse IgG1 |
| Immunogen | E.coli-derived human GNG4 recombinant protein (Position: M1-D52). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 12 kDa |
| UniProt ID | P50150 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ATP synthase D chain mitochondrial antibody; ATP s Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.25-0.5 μg/ml, Mouse, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of GNG4 using anti-GNG4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNG4 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12 kDa. The expected band size for GNG4 is at 12 kDa.
