Anti-Glypican 3 [7H1]

• Catalog Number: orb1906360
• Category: Antibodies
• Description: Anti-Glypican 3 [7H1]
• Target: Glypican 3
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Primate, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).
• UniProt ID: P51654
• Tested applications: ELISA, FC, IHC, WB
• Clone Number: 7H1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX;
• Research Area: Cancer Biology
• Note: For research use only

Immunofluorescence staining of HepG2 cells with anti-Glypican 3 antibody 7H1. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 7H1 (orb1906360) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906360, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Glypican 3 antibody 7H1. HepG2 cells lysate (35 µg protein in RIPA buffer) was resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 7H1 (orb1906360) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.