GFAP Antibody

• Catalog Number: orb43801
• Category: Antibodies
• Description: Mouse Monoclonal to GFAP.
• Target: GFAP
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Conjugation: Unconjugated
• Reactivity: Human, Porcine
• Concentration: 1 mg/ml
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Immunogen: Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
• UniProt ID: P14136
• RRID: AB_10996215
• Tested applications: ICC, IHC-Fr, IHC-P, WB
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: GF-02
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-GFAP antibody
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 2670

Western blotting analysis of human GFAP using mouse monoclonal antibody GF-02 on lysates of Neuro 2a and HEK 293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GFAP monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GFAP at approximately 54 kDa.

Western blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1: immunostaining with anti-GFAP (GF-02); Lane 2: immunostaining with Isotype mouse IgM control (PFR-03; cat. no. orb43568).