gamma-Tubulin GCP2 Antibody

SKU: orb154401

Description

Mouse monoclonal antibody to Gamma-tubulin complex component 2 which is also known as TUBGCP2 or SPBC97, is a ubiquitously expressed 902 amino acid protein that localizes to the centrosome and is involved in microtubule nucleatio. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome.

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone No.: GCP2-01
• Immunogen: GST-fusion protein containing amino acids 2-194 of mouse GCP2
• Target: gamma-Tubulin GCP2
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

GCP-2, GTCC2, GTC2

Western blotting analysis of human gamma-tubulin complex component 2 (GCP2) using mouse monoclonal antibody GCP2-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GCP2 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.

Quick Database Links

Gene Symbol

gamma-Tubulin GCP2

UniProt

Q9BSJ2

NCBI Gene ID

10844