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Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1/500 - 1/1000), IH (1/100 - 1/500) |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Recombinant fusion protein of human Gamma-enolase. The exact sequence is proprietary. |
| Purification | This antibody is purified through a protein G column. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
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![Gamma-enolase Antibody [KO/KD Validated]](/images/pub/media/catalog/product/NewWebsite/16/orb1474030_1.jpg)
Western blot analysis of Gamma-enolase expression in Jurkat (A), CEM (B), U251 MG (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
![Gamma-enolase Antibody [KO/KD Validated]](/images/pub/media/catalog/product/NewWebsite/16/orb1474030_2.jpg)
Immunohistochemical analysis of Gamma-enolase staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
Gamma-enolase Antibody [KO/KD Validated] (orb1474030)
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