GAD65/GAD2 Mouse Monoclonal Antibody

SKU: orb654393

Description

Anti-GAD65/GAD2 Antibody (monoclonal, 4E12). Tested in WB applications. This antibody reacts with Mouse, Rat.

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Mouse, Rat
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 4E12
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid.
• Target: Glutamate decarboxylase 2
• Molecular Weight: 65 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GAD 65; GAD2; GAD65; Glutamate decarboxylase 2

Western blot analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GAD65/GAD2 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAD65/GAD2 at approximately 65 KD. The expected band size for GAD65/GAD2 is at 65 KD.

Quick Database Links

Gene Symbol

Glutamate decarboxylase 2

UniProt

Q05329