Fos B Rabbit Monoclonal Antibody

SKU: orb547788

Description

Anti-Fos B Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Protein Biochemistry

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: DFO-6
• Immunogen: A synthesized peptide derived from human Fos B
• Target: Protein FosB
• Molecular Weight: 45 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein fosB; G0/G1 switch regulatory protein 3; FOSB; G0S3

Immunohistochemical analysis of paraffin-embedded mouse brain, using Fos B Antibody (orb547788) FOSB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FOSB Antibody (orb547788) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of FosB expression in C6 cell lysate treated with serum (orb547788). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB monoclonal antibody (Catalog # orb547788) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

IF analysis of Fos B using anti-Fos B antibody (orb547788) and anti-Beta Tubulin antibody (orb623835). Fos B was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1: 50 with rabbit anti-Fos B Antibody (orb547788) and mouse anti-Beta Tubulin antibody (orb623835) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (orb27705) and DyLight®488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1: 500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Quick Database Links

Gene Symbol

Protein FosB

UniProt

P53539