FOLR1/FRA Antibody

• Catalog Number: orb1806347
• Category: Antibodies
• Description: Anti-FOLR1 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.2 kDa.
• Target: FOLR1 / FRA
• Clonality: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/mL
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Purity: > 95%
• Purification: Protein A
• UniProt ID: P15328
• MW: 150 kDa
• Tested applications: ELISA, FA, FACS, In vivo
• Expression System: CHO
• Endotoxins: < 0.001 EU/ug
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Anti-FOLR1 / FRA Reference Antibody (mirvetuximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-FOLR1 / FRA Reference Antibody (mirvetuximab)is more than 100% ;determined by SEC-HPLC.

Immobilized human FRa His at 2 μg/mL can bind Anti-FOLR1 / FRA Reference Antibody (mirvetuximab);EC50=0.01123μg/mL.

SKOV3 cells were stained with Anti-FOLR1 / FRA Reference Antibody (mirvetuximab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC168=0.03238 ug/mL.

The endocytosis ratio mirvetuximab by Human Frα HEK 293 increased with the increase of antibody concentration; and the Internalization Rate (%) 75% at antibody concentration of 55 ng/mL.