Anti-FIH-1 Antibody

• Catalog Number: orb1473654
• Category: Antibodies
• Description: Mouse monoclonal antibody to FIH-1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FIH-1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/1000 - 1/2000), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIF1AN
• Entrez: 55662
• UniProt ID: Q9NWT6
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: FIH1; Hypoxia-inducible factor 1-alpha inhibitor;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FIH-1 expression in 293 (A), Jurkat (B), ZR751 (C) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)

Immunofluorescent analysis of FIH-1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).