Fibronectin Mouse Monoclonal Antibody

SKU: orb323142

Description

Mouse monoclonal antibody to Fibronectin

Research Area

Cancer, Epigenetics, Immunology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Fibronectin. The exact sequence is proprietary.
• Target: FN1
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CIG antibody, anti Cold insoluble globulin antibody, anti Cold-insoluble globulin antibody, anti ED B antibody, anti fibronectin 1 antibody, anti fibronectin1 antibody, anti fibronectin-1 antibody, anti FINC antibody, anti FN 1 antibody, anti FN antibody, anti FN1 antibody, anti FNZ antibody, anti GFND antibody, anti GFND2 antibody, anti LETS antibody, anti Migration stimulating factor antibody, anti MSF antibody, anti Transformation sensitive protein. antibody, anti Ugl-Y3 antibody

Western blot analysis of Fibronectin expression in Hela (A) whole cell lysates. (Predicted band size: 272 kD; Observed band size: 272 kD)

Immunohistochemical analysis of Fibronectin staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Fibronectin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

FN1

UniProt

P02751, P04937, P11276

NCBI Gene ID

2335, 14268