Anti-EpCAM [KS1/4]

• Catalog Number: orb1089961
• Category: Antibodies
• Description: Mouse monoclonal antibody to EpCAM
• Target: EpCAM
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The original antibody was generated by immunizing BALB/c mice with human adenocarcinoma of the lung cell line UCLA P3.
• UniProt ID: P16422
• Tested applications: ELISA, FC
• Clone Number: KS1/4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell
• Note: For research use only

Flow cytometry using the Anti-EpCAM antibody KS1/4. Paraformaldehyde fixed Caco-2 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of KS1/4 (orb1089960, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Caco-2 cells with anti_EpCAM KS1/4. Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric rabbit IgG version of KS1/4 (orb1089960) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089960, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-EpCAM antibody KS1/4. A431(A), Caco-2(B), MCF7(C) and HepG2(D) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of KS1/4 (orb1089960) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.