Anti-EGFR [C225 (Cetuximab)]

• Catalog Number: orb348862
• Category: Antibodies
• Description: Human monoclonal antibody to EGFR
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
• UniProt ID: P00533
• Tested applications: Blocking, FC
• Clone Number: C225 (Cetuximab)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR, ErbB-1, HER1; epidermal growth factor recept
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Prewett, M. et al. The biologic effects of C225, a chimeric monoclonal antibody to the EGFR, on human prostate carcinoma J Immunother Emphasis Tumor Immunol, 19, 419-427 (1996)

PubMed: 9041461

Reactivity

Human

Binding curve of anti-EGFR antibody to EGFR-Fc fusion protein ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (orb348862).

Immunohistochemical staining of rat stomach tissue using anti-EGFR antibody C225 (Cetuximab) Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (orb348862) was used to stain samples at a concentration of 5 µg/ml.