EGFR Antibody

• Catalog Number: orb1174038
• Category: Antibodies
• Description: Anti-EGFR antibody(DM130), Rabbit mAb
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P00533
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM130
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EGFR;ERBB;ERBB1;HER1;PIG61;mENA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human EGFR protein, His tagged protein orb689439 can bind Rabbit anti-EGFR monoclonal antibody (clone: DM130) in a linear range of 0.06-12 ng/ml.

EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)

Flow cytometry analysis of antigen binding of rabbit anti-human EGFR mAb (orb1174038). (A) orb1174038 does not bind to CHO-S cells that do not express EGFR. (B) A clear peak shift of orb1174038 was seen compared to the control when incubated with EGFR-expressing SNU-5 cells, indicating strong binding of orb1174038 to EGFR. Antibodies were incubated at 5 μg/mL.