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EDNRB Antibody (monoclonal, 15C3)
Catalog Number: orb547585
| Catalog Number | orb547585 |
|---|---|
| Category | Antibodies |
| Description | Anti-EDNRB Antibody (monoclonal, 15C3). Tested in WB applications. This antibody reacts with Human. |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG2b |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human EDNRB, different from the related mouse and rat sequences by one amino acid. |
| UniProt ID | P24530 |
| MW | 45 kDa |
| Tested applications | WB |
| Application notes | Western blot, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500μg/ml |
| Antibody Type | Primary Antibody |
| Clone Number | 15C3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Endothelin receptor type B; ET-B; ET-BR; Endotheli Read more... |
| Note | For research use only |
Western blot analysis of EDNRB using anti-EDNRB antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EDNRB antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EDNRB at approximately 45KD. The expected band size for EDNRB is at 45KD.
