Anti-dsRNA monoclonal antibody K1

• Catalog Number: orb653832
• Category: Antibodies
• Description: Anti-dsRNA monoclonal antibody K1 can be used for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. It is recommended for Poly I:C detection (higher affinity than J2) and Anti-dsRNA monoclonal antibody J2 alternative to resolve cross-reactions and/or remove background in those rare experimental setups where J2 does not provides satisfactory results.
• Clonality: Monoclonal
• Form/Appearance: lyophilised from a 1 mg/ml solution in PBS
• Conjugation: Unconjugated
• Solubility (25°C): To prepare a 1 µg/µl PBS antibody solution add 200 µl (orb653832) or 500 µl (orb1733495) sterile, DNAse/RNAse-free distilled water. As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend to spin down (microcentrifuge) the reconstituted antibody before use, and to use the supernatant only.
• Storage: store at -20 °C to -80 °C upon reconstitution for long-term storage
• Note: For research use only
• Application notes: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immuno-electron microscopy. Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.
• Expiration Date: 12 months from date of receipt.