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Anti-DDX5 (pY593) Antibody

Catalog Number: orb1422530

DispatchUsually dispatched within 5-10 working days
$ 340.00
Catalog Numberorb1422530
CategoryAntibodies
DescriptionRabbit polyclonal antibody to DDX5 (pY593).
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
UniProt IDP17844
Tested applicationsIF, IHC, WB
Dilution rangeWB: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Clone NumberDDX5
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesG17P1; HELR; HLR1; Probable ATP-dependent RNA heli
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NoteFor research use only
Anti-DDX5 (pY593) Antibody

Immunofluorescent analysis of DDX5 (pY593) staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Anti-DDX5 (pY593) Antibody

Immunohistochemical analysis of DDX5 (pY593) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-DDX5 (pY593) Antibody

Western blot analysis of DDX5 (pY593) expression in C6 (A), EC9706 (B) whole cell lysates.