Anti-DDDDK-tag [M2.1]

SKU: orb396135

Description

Mouse monoclonal antibody to DDDDK-tag.

Images & Validation

• Tested Applications: ELISA, IF, WB

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: M2.1
• Immunogen: Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.
• Target: DDDDK-tag
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

FLAG-tag; Equivalent to FLAG antibodies from Sigma; DYKDDDDK; DDDK; AspTyrLysAspAspAspAspLy

Binding of Anti-DDDDK antibody to Multitag protein Comparison of the binding of the anti-DDDDK antibody M2.1 (orb396135) and an equivalent antibody M2 from a competitor to Multitag protein. Wells were pre-coated with 5 ug/mg Multitag protein and then probed with serial dilutions of antibody followed by an HRP-conjugated goat anti-mouse antibody.

Binding curves of the rabbit IgG chimeric version of the Anti-DDDDK-tag antibody M2.1 (orb396143; blue line) and isotype control (orb256390, anti-fluorescein; orange line) to an ELISA plate coated with Anti-i-motif DNA [iMab] at a concentration of 2.5 ug/ml.

Quick Database Links

Gene Symbol

DDDDK-tag

References

Brizzard, B. L. et al. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution Biotechniques, 16, 730-735 (1994)

PubMed: 8024796