Cytokeratin 5 Mouse Monoclonal Antibody

SKU: orb865591

Description

Anti-Cytokeratin 5 Antibody (monoclonal, B1J4H0). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x^6 cells, Human
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: B1J4H0
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
• Target: Keratin, type II cytoskeletal 5
• Molecular Weight: 62 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

58 kDa cytokeratin; Cell and organelle markers; CK 5; CK5; Cytokeratin 5; Cytoskeleton Marker; K5; keratin 5; KRT5; KRT5A; Type II keratin Kb5

Quick Database Links

Gene Symbol

Keratin, type II cytoskeletal 5

UniProt

P13647