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Anti-CPSF6 Antibody (monoclonal, 3F11E1)

Catalog Number: orb1184752

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1184752
CategoryAntibodies
DescriptionAnti-CPSF6 Antibody (monoclonal, 3F11E1). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Species/HostMouse
ClonalityMonoclonal
Clone Number3F11E1
Tested applicationsFC, ICC, IF, IHC, WB
ReactivityHuman, Monkey, Mouse, Rat
IsotypeMouse IgG1
ImmunogenE.coli-derived human CPSF6 recombinant protein (Position: R50-Q176).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW68 kDa
UniProt IDQ16630
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCoatomer subunit epsilon; Epsilon-coat protein; Ep
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

Flow Cytometry analysis of HepG2 cells using anti-CPSF6 antibody. Overlay histogram showing HepG2 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CPSF6 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IF analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL mouse anti-CPSF6 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

IHC analysis of CPSF6 using anti-CPSF6 antibody. CPSF6 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-CPSF6 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.

Anti-CPSF6 Antibody (monoclonal, 3F11E1)

Western blot analysis of CPSF6 using anti-CPSF6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CPSF6 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CPSF6 at approximately 68 kDa. The expected band size for CPSF6 is at 59 kDa.

  • Anti-CPSF6 Antibody Picoband (monoclonal, 3F11E1) [orb1882110]

    FC,  ICC,  IF,  IHC,  WB

    Human, Monkey, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • Anti-CPSF6 Antibody (monoclonal, 3F11E1) [orb2594010]

    FC,  ICC,  IF,  IHC,  WB

    Human, Monkey, Mouse, Rat

    Mouse

    Monoclonal

    iFluor647

    100 μg
  • Anti-CPSF6 Antibody (monoclonal, 3F11E1) [orb2594011]

    FC,  ICC,  IF,  IHC,  WB

    Human, Monkey, Mouse, Rat

    Mouse

    Monoclonal

    PE

    100 μg
  • Anti-CPSF6 Antibody (monoclonal, 3F11E1) [orb2594012]

    FC,  ICC,  IF,  IHC,  WB

    Human, Monkey, Mouse, Rat

    Mouse

    Monoclonal

    APC

    100 μg
  • Anti-CPSF6 Antibody (monoclonal, 3F11E1) [orb2594013]

    FC,  ICC,  IF,  IHC,  WB

    Human, Monkey, Mouse, Rat

    Mouse

    Monoclonal

    HRP

    100 μg