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Description
Research Area
Cancer Biology, Cardiovascular Research; Musculoskeletal & Connective Tissue Research, Cell Biology, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
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Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunofluorescence, 2μg/ml |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | B5I5 |
| Immunogen | E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV. |
| Target | Collagen alpha-1 |
| Molecular Weight | 220 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−collagen type IV alpha 1 chain; BSVD; BSVD1s; PADMAL; RATOR; COL4A1
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Quick Database Links
Gene Symbol
Collagen alpha-1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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