Anti-CD98 [EP3-1]

• Catalog Number: orb1152499
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD98
• Target: CD98
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. CD98-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
• UniProt ID: Q01650
• Tested applications: IF, IP
• Clone Number: EP3-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LAT1; E16; MPE16; SLC7A5
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only

Flow cytometry using the Anti-CD98 antibody EP3-1. Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of EP3-1 (orb1152499, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with antiCD98 EP3-1. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (orb1152499) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152499, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.