Anti-CD6 [OX-126]

SKU: orb2645750

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Isotype: Rabbit IgG, Kappa
• Clone No.: OX-126
• Immunogen: This antibody was raised by immunizing BALB/c mice with a fusion protein consisting of human CD6 domain 3 and rat CD4 domains 3 and 4 (CD6d3CD4d3 + 4) (corrected, see: (Hassan et al., 2019; pmid: 30890626)).
• Target: CD6
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

TP120; OX126; MRC OX-126; MRC OX 126

Flow cytometry using the Anti-CD6 antibody OX-126. Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of OX-126 (orb2645751, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of human peripheral blood leukocytes using anti-CD6 OX-126. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes stained with the chimeric mouse IgG version of OX-126 (orb2645751) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645751, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD6

UniProt

P30203