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Catalog Number | orb411573 |
---|---|
Category | Antibodies |
Description | Human monoclonal antibody to CD55 |
Species/Host | Human |
Clonality | Monoclonal |
Clone Number | LU30 |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | Human IgG1 |
Immunogen | Human CD55. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | CD55 |
UniProt ID | P08174 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | DAF; Complement decay-accelerating factor Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30 U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (orb411574, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.
Immunohistochemical staining of human lung tissue using anti-CD55 antibody LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (orb411574). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb411574 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3, 3′-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 µg/ml.