Cart summary

You have no items in your shopping cart.

Anti-CD55 [LU30]

Catalog Number: orb411573

DispatchUsually dispatched within 5-10 working days
$ 530.00
Catalog Numberorb411573
CategoryAntibodies
DescriptionHuman monoclonal antibody to CD55
Species/HostHuman
ClonalityMonoclonal
Clone NumberLU30
Tested applicationsELISA, IHC, WB
ReactivityHuman
IsotypeHuman IgG1
ImmunogenHuman CD55.
Concentration1 mg/ml
PurityPurified
ConjugationUnconjugated
TargetCD55
UniProt IDP08174
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namesDAF; Complement decay-accelerating factor
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CD55 [LU30]

Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30 U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 (orb411574, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Anti-CD55 [LU30]

Immunohistochemical staining of human lung tissue using anti-CD55 antibody LU30 Anti-CD55 (Complement decay-accelerating factor)staining of paraffin embedded human lung tissue using the rabbit-chimeric version of LU30 (orb411574). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb411574 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3, 3′-diaminobenzidine), and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration, 2-4 µg/ml.

  • Anti-CD55 [LU30] [orb411574]

    ELISA,  IHC,  WB

    Human

    Human

    Monoclonal

    Unconjugated

    0.2 mg