Anti-CD27 (varlilumab biosimilar) mAb

• Catalog Number: orb689323
• Category: Antibodies
• Description: Anti-CD27 (varlilumab biosimilar) mAb
• Species/Host: Human
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Biosimilar Antibody
• Dilution range: ELISA 1:5000-10000|Flow Cyt 1:100
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: CD27
• UniProt ID: P26842
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD27;TNFRSF7;S152;T14;Tp55
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (orb689459) can bind Anti-CD27 Neutralizing antibody in a linear range of 3.2-80.0 ng/ml. In order to specifically detect orb689323, mouse anti-human Fab-specific antibody was used as detection antibody.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, mFc-His tagged protein orb689374 can bind Anti-CD27 Neutralizing antibody in a linear range of 0.64-16.0 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human CD27 protein (A) were surface stained with anti-CD27 neutralizing antibody 1 μg/ml (varlilumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.