Anti-CD235a Biotin

• Catalog Number: orb1088488
• Category: Antibodies
• Description: CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.
• Clonality: Monoclonal
• Clone Number: JC159
• Tested applications: FC, IHC-Fr, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1
• Immunogen: Membrane preparation from splenic hairy cell leukemia
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD235a
• Entrez: 2993
• UniProt ID: P02724
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Glycophorin A antibody, anti-GYPA antibody, a
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of human CD235a positive erythrocytes (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin APC).