Anti-CD20 Reference Antibody (rituximab)

• Catalog Number: orb1806379
• Category: Antibodies
• Description: Anti-CD20 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 146.32 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 146.32 kDa
• Target: CD20
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD20 Reference Antibody (rituximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CD20 Reference Antibody (rituximab)is more than 98.97% ;determined by SEC-HPLC.

Immobilized human EGFR His at 2 μg/mL can bind Anti-CD20 Reference Antibody (rituximab);EC50=0.01573/0.01258μg/mL.

Raji cells were stained with Anti-CD20 Reference Antibody (rituximab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC87=1.334 μg/mL.

Anti-CD20 Reference Antibody (rituximab)-induced CDC activity was evaluated using Raji Cell. The max Lysis rate was approximately 74%.

Anti-CD20 Reference Antibody (rituximab)-induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.