Anti-CD19 [FMC63]

• Catalog Number: orb348921
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD19
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FMC63
• Tested applications: Depletion, FACS, FC, IF, IHC, IP, In vivo
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Human prolymphocytic leukaemia cell line JVM3.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD19
• UniProt ID: P15391
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B-lymphocyte antigen CD19; B-lymphocyte surface an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Nicholson, I. C. et al. Construction and characterisation of a functional CD19 specific single chain Fv fragment for immunotherapy of B lineage leukaemia and lymphoma Mol Immunol, 34, 1157-1165 (1997)

PubMed: 9566763

Reactivity

Human

Flow-cytometry using anti-CD19 antibody FMC63 Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of FMC63 (orb348921, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of rat spleen tissue using anti-CD19 antibody FMC63. Anti-CD19 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The mouse IgG 2A-chimeric version of FMC63 (orb348877) was used to stain samples at a concentration of 3 µg/ml.