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Anti-CD166 antibody(DMC682), IgG1 Chimeric mAb

Catalog Number: orb1826955

DispatchUsually dispatched within 5-10 working days
$ 150.00
Catalog Numberorb1826955
CategoryAntibodies
DescriptionAnti-CD166 antibody(DMC682); IgG1 Chimeric mAb
Species/HostRabbit
ClonalityMonoclonal
Clone NumberDMC682
Tested applicationsFC
ReactivityHuman
IsotypeRabbit/Human Fc chimeric IgG1
Antibody TypeRecombinant Antibody
Dilution rangeFlow Cyt 1:100
ConjugationUnconjugated
TargetCD166
UniProt IDQ13740
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCD166; MEMD
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NoteFor research use only
Application notesLyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Expiration Date12 months from date of receipt.
Anti-CD166 antibody(DMC682), IgG1 Chimeric mAb

CD166 protein is expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD166 on Expi293 cells transfected with human CD166 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Anti-CD166 antibody(DMC682), IgG1 Chimeric mAb

Flow cytometry analysis of antigen binding of anti-human CD166 mAb (orb1826955). (A) orb1826955 does not bind to K562 cells that do not express CD166. (B) A clear peak shift of orb1826955 was seen compared to the control when incubated with CD166-expressing Hela cells, indicating strong binding of orb1826955 to CD166. Antibodies were incubated at 5 μg/mL.

Anti-CD166 antibody(DMC682), IgG1 Chimeric mAb

Flow cytometry analysis of antigen binding of anti-human CD166 mAb (orb1826955). (A) orb1826955 does not bind to K562 cells that do not express CD166. (B) A clear peak shift of orb1826955 was seen compared to the control when incubated with CD166-expressing Huh7 cells, indicating strong binding of orb1826955 to CD166. Antibodies were incubated at 5 μg/mL.