Anti-CD166 antibody(DMC682), IgG1 Chimeric mAb

• Catalog Number: orb1826955
• Category: Antibodies
• Description: Anti-CD166 antibody(DMC682); IgG1 Chimeric mAb
• Target: CD166
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit/Human Fc chimeric IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• UniProt ID: Q13740
• Tested applications: FC
• Dilution range: Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DMC682
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD166; MEMD
• Note: For research use only

CD166 protein is expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD166 on Expi293 cells transfected with human CD166 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of anti-human CD166 mAb (orb1826955). (A) orb1826955 does not bind to K562 cells that do not express CD166. (B) A clear peak shift of orb1826955 was seen compared to the control when incubated with CD166-expressing Hela cells, indicating strong binding of orb1826955 to CD166. Antibodies were incubated at 5 μg/mL.

Flow cytometry analysis of antigen binding of anti-human CD166 mAb (orb1826955). (A) orb1826955 does not bind to K562 cells that do not express CD166. (B) A clear peak shift of orb1826955 was seen compared to the control when incubated with CD166-expressing Huh7 cells, indicating strong binding of orb1826955 to CD166. Antibodies were incubated at 5 μg/mL.