Anti-CD16 Antibody

• Catalog Number: orb323117
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD16
• Target: FCGR3A; FCGR3B
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human CD16. The exact sequence is proprietary.
• UniProt ID: O75015, P08637
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:100-1:200
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FCGR3A antibody, anti CD16A antibody, anti FC
• Note: For research use only
• Entrez: 2214, 2215

Western blot analysis of CD16 expression in Jurkat (A), K562 (B) whole cell lysates. (Predicted band size: 29; 26 kD; Observed band size: 45 kD)

Immunohistochemical analysis of CD16 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.