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Item 1 of 3
Anti-CD127 (pY449) Antibody
Catalog Number: orb1421921
| Catalog Number | orb1421921 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to CD127 (pY449). |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | IL7R |
| Tested applications | IF, WB |
| Reactivity | Human |
| Dilution range | WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), E: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
| Conjugation | Unconjugated |
| UniProt ID | P16871 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Interleukin-7 receptor subunit alpha; IL-7 recepto Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD127 (pY449) expression in HCT116 (A), HEK293T (B) whole cell lysates.
Direct ELISA antibody dose-response curve using Anti-CD127 (pY449) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Immunofluorescent analysis of CD127 (pY449) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
