CBS Antibody (monoclonal, 5B5D7)

SKU: orb1184738

Description

Anti-CBS Antibody (monoclonal, 5B5D7). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 5B5D7
• Immunogen: E.coli-derived human CBS recombinant protein (Position: K102-E342).
• Molecular Weight: 61 kDa
• Purification: Immunogen affinity purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Cytosolic NADP isocitrate dehydrogenase antibody; Cytosolic NADP-isocitrate dehydrogenase antibody; Epididymis luminal protein 216 antibody; Epididymis secretory protein Li 26 antibody; HEL-216 antibody; HEL-S-26 antibody; ICDH antibody; IDCD antibody; IDH antibody; IDH1 antibody; IDHC_HUMAN antibody; IDP antibody; IDPC antibody; Isocitrate dehydrogenase [NADP] cytoplasmic antibody; Isocitrate dehydrogenase 1 (NADP+) soluble antibody; NADP dependent isocitrate dehydrogenase cytosolic antibody; NADP dependent isocitrate dehydrogenase peroxisomal antibody; NADP (+)-specific ICDH antibody; Oxalosuccinate decarboxylase antibody; PICD antibody

Flow Cytometry analysis of MCF-7 cells using anti-CBS antibody. Overlay histogram showing MCF-7 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CBS Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of CBS using anti-CBS antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CBS antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CBS at approximately 61 kDa. The expected band size for CBS is at 61 kDa.

Quick Database Links

UniProt

P35520