Caspase 5 CASP5 Rabbit Monoclonal Antibody

SKU: orb548357

Description

Caspase 5 CASP5 Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Reactivity: Human
• Application Notes:
  WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200IP 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: GOI-3
• Immunogen: A synthesized peptide derived from human Caspase 5
• Molecular Weight: 50 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Caspase-5; CASP-5; 3.4.22.58; ICE (rel)-III; Protease ICH-3; Protease TY; Caspase-5 subunit p20; Caspase-5 subunit p10; CASP5; ICH3

Western blot analysis of Caspase 5 expression in HeLa cell lysate (orb548357). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP5 monoclonal antibody (Catalog # orb548357) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

IHC analysis of Caspase 5 using anti-Caspase 5 antibody (orb548357) on human breast cancer. Caspase 5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase 5 Antibody (orb548357) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Quick Database Links

UniProt

P51878