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Description
Research Area
Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells |
| Reactivity | Human, Monkey, Mouse |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | B0K8 |
| Immunogen | E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively. |
| Target | Calpain-2 catalytic subunit |
| Molecular Weight | 80 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Calpain 2; Calpain 2 catalytic subunit; Calpain 2 large subunit; Calpain large polypeptide L2; Calpain M type; CANP 2; CANP2; CANPL2; CANPml; CAPN2; M calpain; mCANP; Millimolar calpain
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Quick Database Links
Gene Symbol
Calpain-2 catalytic subunit
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry













