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Anti-C3a [YHP133]
Description
Research Area
Images & Validation
−| Reactivity | Human |
|---|
Key Properties
−| Host | Human |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | YHP133 |
| Immunogen | The original antibody was generated using human C3a as an immunogen. |
| Target | C3a |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot using anti-C3a antibody YHP133. Human serum (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the mouse version of YHP133 at 0.3 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence. During testing, a non-specific 26kDa band was observed; further optimisation of assay conditions may be required.
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Anti-C3a [YHP133] (orb1906403)
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