c-FOS Antibody

• Catalog Number: orb323121
• Category: Antibodies
• Description: The c-FOS Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human c-FOS. Purification: Affinity chromatography
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: Recombinant protein corresponding to human c-FOS.
• UniProt ID: P01100
• Tested applications: IF, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: G0S7; Proto-oncogene c-Fos; Cellular oncogene fos;
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 2353

Western blot analysis of c-FOS expression in Hela (A), 293T (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 62 kD)

Immunohistochemical analysis of c-FOS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.