BrdU Antibody

• Catalog Number: orb44454
• Category: Antibodies
• Description: Mouse monoclonal antibody to 5-BrdU
• Target: BrdU
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: 5-bromodeoxyuridine conjugated with hemocyanine.
• RRID: AB_10994048
• Tested applications: FC, ICC, IHC-P
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.Immunocytochemistry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Excellent.
• Antibody Type: Primary Antibody
• Clone Number: MoBu-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 5-bromodeoxyuridine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1).