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Anti-BrdU Purified
Catalog Number: orb44454
| Catalog Number | orb44454 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to 5-BrdU |
| Target | BrdU |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified by protein-A affinity chromatography. |
| Immunogen | 5-bromodeoxyuridine conjugated with hemocyanine. |
| RRID | AB_10994048 |
| Tested applications | FC, ICC, IHC-P |
| Application notes | Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.Immunocytochemistry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Excellent. |
| Antibody Type | Primary Antibody |
| Clone Number | MoBu-1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti Bromodeoxyuridine antibody, anti BUdr antibod Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.
Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1).
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