Ig Light Chains Antibody

SKU: orb44871

Description

Mouse Monoclonal to Bovine IgG (L chain).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC-Fr, WB
• Reactivity: Bovine, Sheep
• Application Notes:
  Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: IVA285-1
• Immunogen: Fraction of Bovine immunoglobulin.
• Target: Ig Light Chains
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Immunoglobulin light chains

Quick Database Links

Gene Symbol

Ig Light Chains

NCBI Gene ID

35