Ig Light Chains Antibody

SKU: orb44871

Description

Mouse Monoclonal to Bovine IgG (L chain).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC-Fr, WB
• Reactivity: Bovine, Sheep
• Application Notes:
  Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: IVA285-1
• Immunogen: Fraction of Bovine immunoglobulin.
• Target: Ig Light Chains
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Immunoglobulin light chains

Western blotting detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).

Separation of bovine Ig Light Chains positive leukocytes (red-filled) from Ig Light Chains negative leukocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Quick Database Links

Gene Symbol

Ig Light Chains

NCBI Gene ID

35