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Item 1 of 3
Anti-Bov Ig Light Chains Purified
Catalog Number: orb44871
| Catalog Number | orb44871 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Bovine IgG (L chain). |
| Target | Ig Light Chains |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Sheep |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified by protein-A affinity chromatography. |
| Purification | Purified by protein-A affinity chromatography. |
| Immunogen | Fraction of Bovine immunoglobulin. |
| RRID | AB_10990179 |
| Tested applications | FC, IHC-Fr, WB |
| Application notes | Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 µg/ml |
| Antibody Type | Primary Antibody |
| Clone Number | IVA285-1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-IgG light chain antibody |
| Note | For research use only |
Western blotting detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).
Separation of bovine Ig Light Chains positive leukocytes (red-filled) from Ig Light Chains negative leukocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 µg/ml, GAM APC).
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine Ig Light Chains (IVA285-1) purified antibody (concentration in sample 3 µg/ml, GAM APC).
