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Catalog Number | orb43890 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to CD18. |
Clonality | Monoclonal |
Clone Number | IVA35 |
Tested applications | FC, ICC, IP |
Reactivity | Bovine, Human |
Isotype | Mouse IgG1 |
Immunogen | Bovine leukocytes. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD18 |
Entrez | 281877 |
UniProt ID | P32592 |
RRID | AB_10992725 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CD18 (bovine) antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of bovine lymphocytes stained using anti-bovine CD18 (IVA35) purified antibody (concentration in sample 10 μg/ml, GAM APC, red-filled) from bovine lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD18 (IVA35) purified antibody (concentration in sample 10 μg/ml) GAM APC.
Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD18 (IVA35); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).