Anti-BMI1 Antibody

• Catalog Number: orb1473910
• Category: Antibodies
• Description: Mouse monoclonal antibody to BMI1
• Target: BMI1
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: Recombinant fusion protein of human BMI1. The exact sequence is proprietary.
• UniProt ID: P35226
• Tested applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PCGF4; RNF51; Polycomb complex protein BMI-1; Poly
• Note: For research use only
• Entrez: 648, 100532731

Western blot analysis of BMI1 expression in Hela (A), A549 (B) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 43 kD)

Immunofluorescent analysis of BMI1 staining in NCIH460 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).