beta Actin ACTB Rabbit Monoclonal Antibody

SKU: orb547686

Description

Anti-beta Actin ACTB Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:2000-20000IHC 1:50-200ICC/IF 1:50-200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: BF-1
• Immunogen: A synthesized peptide derived from human beta Actin
• Molecular Weight: 42 kDa
• Purification: Affinity-chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB

Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate.

Immunohistochemical analysis of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart, using beta Actin Antibody.

Immunofluorescent analysis of HeLa cells, using beta Actin Antibody.

Quick Database Links

UniProt

P60709