Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab)

• Catalog Number: orb1818284
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human, Monkey
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 145 Da
• UniProt ID: Q9NZQ7
• Protein Sequence: B7-H1 / PD-L1 / CD274
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Programmed cell death 1 ligand 1, PD-L1, PDCD1 lig
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) is more than 100%, determined by SEC-HPLC.

Immobilized human PD L1 His at 2 µg/mL can bind Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab), EC50=0.005894 µg/mL

Atezolizumab inhibited the tumor growth of MC38 on C57BL/6N mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 85.2% at 2 mpk at D35.

The endocytosis ratio atezolizumab by HCC827 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 0.5 nM.

Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Human PD-L1 CHO-K cells were stained with Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (atezolizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC263=1.533 nM