Anti-ANXA4 [SAIC-14C-10F12]

SKU: orb1089996

Description

Rabbit monoclonal antibody to ANXA4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: SAIC-14C-10F12
• Immunogen: Peptide "DEGNYLDDALVR" derived from Annexin A4 conjugated to KLH.
• Target: ANXA4
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANXA4 protein

Flow cytometry using the Anti-ANXA4 antibody SAIC-14C-10F12. Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-14C-10F12 (orb1089996, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HepG2 cells with anti-ANXA4 SAIC-14C-10F12. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-14C-10F12 (orb1089996) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089996, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-ANXA4 antibody SAIC-14C-10F12. HepG2(A) (0.001 µg/ml), A431(B) (0.001 µg/ml), and HeLa(C) (0.01 µg/ml) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-14C-10F12 (orb1089996), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

ANXA4

UniProt

P09525