Alpha A Crystallin Antibody (monoclonal, 10B9)

SKU: orb763150

Description

Anti-Alpha A Crystallin Antibody (monoclonal, 10B9). Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: Western blot, 0.25-0.5 μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 10B9
• Immunogen: E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.
• Target: Hepatocyte growth factor receptor
• Molecular Weight: 20-23 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

CRYAA; Acry 1; CRYA 1; CRYA1; CRYAA; Crystallin alpha 1; Crystallin alpha A; HSPB 4

IF analysis of Alpha A Crystallin using anti-Alpha A Crystallin antibody. Alpha A Crystallin was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL mouse anti-Alpha A Crystallin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of Alpha A Crystallin using anti-Alpha A Crystallin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Alpha A Crystallin antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Alpha A Crystallin at approximately 20-23 kDa. The expected band size for Alpha A Crystallin is at 20-23 kDa.

Quick Database Links

Gene Symbol

Hepatocyte growth factor receptor

UniProt

P02489