ACTN2 Rabbit Polyclonal Antibody

SKU: orb412196

Description

The ACTN2 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human ACTN2 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human ACTN2
• Target: ACTN2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein

Western blot analysis of ACTN2 expression in HT29 (A), Hela (B), mouse heart (C), mouse lung (D), rat liver (E) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 104 kD)

Immunofluorescent analysis of ACTN2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

ACTN2

UniProt

P35609, Q9JI91

NCBI Gene ID

11472, 88