Anti-ACTN1 Antibody

• Catalog Number: orb323205
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACTN1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Mouse, Rat
• Immunogen: Recombinant protein corresponding to mouse ACTN1.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ACTN1
• Entrez: 81634, 109711
• UniProt ID: Q9Z1P2, Q7TPR4
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Alpha-actinin-1 antibody, anti Alpha-actinin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ACTN1 expression in NIH3T3 (A), PC12 (B), rat heart (C) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 100 kD)

Immunohistochemical analysis of ACTN1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.